High Performance Liquid Chromatography (HPLC) separation technique is widely used in her lab chip research. Separation of several neurotransmitters such as Dopamine, Serotonin and Epinephrine were performed using HPLC and were detected using post column electrochemical detection using conventional HPLC instrumentation. In-column electrochemical detection (ICED) for these neurotransmitter were performed using the Liquid Chromatography Lab Chip.

Physical model simulation study using Comsol Multiphysics. Comsol Multiphysics is a software program that create an simulation environment to allow better understanding of finite element analysis and physics-based system. Hydrodynamic properties and electrochemical reactions within microchannel are studied. Figure shows that the numerical models that was built according to the device specification to understand the feasibility and differences of electrochemical detection within a packed column for a liquid chromatography.

Microfluidic devices or Lab on a Chip (LOC) is microdevices with wide range of application from sample preparation, analytical chemistry, environmental analysis to biomedical analysis. LOC devices was first indicated by "Micro Total Analysis Systems" (Manz et al., 1990). Many fabrication techniques are reported and the microfluidic research is emerging and growing rapidly replacing conventional laboratory facilities. Lab on a chip has increasingly important for chemical analysis, for instance, in environment, medical, genomic or proteomic studies. Chromatography and electrophoresis separation techniques are widely occupied in these lab chips in order to perform separation of complex mixture of chemicals. The designs of the chip and the compatibility of the material used for the fabrication are crucial. Polymers such as Silicons, acrylic and polyester are normally the substrate used for the fabrication. However, glass also a very useful material but depends on the application and techniques used in the chip. Figure shows the whole column detection for liquid chromatography separation process.